Packed Bed Adsorption (PBA) Chromatography Procedure (Simplified)

Overview

A Simplified Packed Bed Adsorption (PBA) Chromatography Procedure can be created by selecting the following menu option:
Unit Procedures } Chromatography/Adsorption } PBA Chromatography (Simplified

PBA_Simp_Column.bmp

 

Operating Mode

Batch/Semi-Continuous

Host Equipment

Packed Bed Adsorption (PBA) Chromatography Column

Emissions Port (Vent)

No

Special Input/Output Ports

No

 

 

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This unit procedure simulates any type of adsorptive chromatography (e.g., ion exchange, affinity, HIC, reverse phase, etc.) that takes place in a packed bed column in a simplified manner.

Special Inputs / Outputs

None.

Operations

The following operation types are currently supported in the context of this unit procedure:

●      Load, see Packed Bed Adsorption (PBA) Column Loading (Simplified)

●      Wash, see Column Wash (Simplified)

●      Elute, see Column Elution (Simplified)

●      Regenerate, see Column Regeneration (Simplified)

●      Equilibrate, see Column Equilibration (Simplified)

●      CIP, see Clean-In-Place (CIP)

●      Hold, see Holding

●      SIP, see Steam-In-Place (SIP)

SEe Also

The following procedures offer a similar functionality:

●      Gel Filtration Procedure

●      Packed Bed Adsorption (PBA) Chromatography Procedure (Detailed) in Bind-and-Elute Mode

●      Packed Bed Adsorption (PBA) Chromatography Procedure (Detailed) in Flow-Through Mode

●      Expanded Bed Adsorption (EBA) Chromatography Procedure

●      Membrane Adsorption Procedure in Flow-Through Mode

●      Membrane Adsorption Procedure in Bind-and-Elute Mode