Packed Bed Adsorption (PBA) Chromatography Procedure (Simplified)

Overview

A Simplified Packed Bed Adsorption (PBA) Chromatography Procedure can be created by selecting the following menu option:
Unit Procedures } Chromatography/Adsorption } PBA Chromatography (Simplified

PBA_Simp_Column.bmp

 

Operating Mode

Batch/Semi-Continuous

Host Equipment

Packed Bed Adsorption (PBA) Chromatography Column

Emissions Port (Vent)

No

Special Input/Output Ports

No

 

 

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This unit procedure simulates any type of adsorptive chromatography (e.g., ion exchange, affinity, HIC, reverse phase, etc.) that takes place in a packed bed column in a simplified manner.

Special Inputs / Outputs

None.

Operations

The following operation types are currently supported in the context of this unit procedure:

      Load, see Packed Bed Adsorption (PBA) Column Loading (Simplified)

      Wash, see Column Wash (Simplified)

      Elute, see Column Elution (Simplified)

      Regenerate, see Column Regeneration (Simplified)

      Equilibrate, see Column Equilibration (Simplified)

      CIP, see Clean-In-Place (CIP)

      Hold, see Holding

      SIP, see Steam-In-Place (SIP)

SEe Also

The following procedures offer a similar functionality:

      Gel Filtration Procedure

      Packed Bed Adsorption (PBA) Chromatography Procedure (Detailed) in Bind-and-Elute Mode

      Packed Bed Adsorption (PBA) Chromatography Procedure (Detailed) in Flow-Through Mode

      Expanded Bed Adsorption (EBA) Chromatography Procedure

      Membrane Adsorption Procedure in Flow-Through Mode

      Membrane Adsorption Procedure in Bind-and-Elute Mode