The main objective of this operation is to estimate the time and buffer required for washing (or equilibrating/rinsing/regenerating) a column.
● Packed Bed Adsorption (PBA) Chromatography Procedure (Simplified)
● Expanded Bed Adsorption (EBA) Chromatography Procedure
The buffer volume is equal to the bed volume of the column times the volume factor (in packed bed volumes) that is specified by the user.
In terms of elution flowrate you can either specify the linear velocity, the absolute flowrate, or the relative flowrate:
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The process time is calculated using the following equation:
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The feed (buffer) volume in the above equation is expressed in ‘packed bed volumes’.
All materials used for washing/equilibrating/regenerating a column end up in the waste stream.
Note. Since the flowrate of a wash/equilibration/regeneration stream is adjusted by the model, the user must make sure that the composition of the stream is correct and not worry about the total stream flowrate.
The interface of this operation has the following tabs:
● Oper. Cond’s, see Column Wash (Simplified): Oper. Conds Tab
● Labor, etc, see Operations Dialog: Labor etc. Tab
● Description, see Operations Dialog: Description Tab
● Batch Sheet, see Operations Dialog: Batch Sheet Tab
● Scheduling, see Operations Dialog: Scheduling Tab
