Packed Bed Adsorption (PBA) Chromatography Procedure (Detailed) in Flow-Through Mode

Overview

A Packed Bed Adsorption (PBA) Chromatography Procedure can be created by selecting the following menu option:
Unit Procedures } Chromatography/Adsorption } PBA Chromatography (Detailed) } in Flow-Through Mode

PBA_Column_FT.bmp

 

Operating Mode

Batch/Semi-Continuous

Host Equipment

Packed Bed Adsorption (PBA) Chromatography Column

Emissions Port (Vent)

No

Special Input/Output Ports

No

 

 

This unit procedure simulates any type of adsorptive chromatography (e.g., ion exchange, affinity, HIC, reverse phase, etc.) that takes place in flow-through mode in a packed bed column. The procedure can be executed in a batch/semi continuous mode only.

Special Inputs / Outputs

None.

Operations

The following operation types are currently supported in the context of this unit procedure:

      Equilibrate, see Column Equilibration

      Flush, see Column Flush

      Flow Through, see Packed Bed Adsorption (PBA) Column Flow-Through

      Hold, see Holding

      Regenerate, see Column Regeneration

      Sanitize, see Column Sanitization

      Strip, see Column Strip

SEe Also

The following procedures offer a similar functionality:

      Gel Filtration Procedure

      Packed Bed Adsorption (PBA) Chromatography Procedure (Simplified)

      Packed Bed Adsorption (PBA) Chromatography Procedure (Detailed) in Bind-and-Elute Mode

      Expanded Bed Adsorption (EBA) Chromatography Procedure

      Membrane Adsorption Procedure in Flow-Through Mode

      Membrane Adsorption Procedure in Bind-and-Elute Mode