Packed Bed Adsorption (PBA) Chromatography Procedure (Detailed) in Bind-and-Elute Mode

Overview

A Packed Bed Adsorption (PBA) Chromatography Procedure in Bind-and-Elute Mode can be created by selecting the following menu option:
Unit Procedures } Chromatography/Adsorption } PBA Chromatography (Detailed) } in Bind-and-Elute Mode

	Operating Mode	Batch/Semi-Continuous
	Host Equipment	Packed Bed Adsorption (PBA) Chromatography Column
	Emissions Port (Vent)	No
	Special Input/Output Ports	No

This unit procedure simulates any type of adsorptive chromatography (e.g., ion exchange, affinity, HIC, reverse phase, etc.) that takes place in bind-and-elute mode in a packed bed column. The procedure can be executed in a batch/semi continuous mode only.

Special Inputs / Outputs

None.

Operations

The following operation types are currently supported in the context of this unit procedure:

●      Elute, see Column Elution

●      Equilibrate, see Column Equilibration

●      Flush, see Column Flush

●      Hold, see Holding

●      Load, see Packed Bed Adsorption (PBA) Column Loading

●      Regenerate, see Column Regeneration

●      Sanitize, see Column Sanitization

●      Wash, see Column Elution: Elution Strategy Tab

SEe Also

The following procedures offer a similar functionality:

●      Gel Filtration Procedure

●      Packed Bed Adsorption (PBA) Chromatography Procedure (Simplified)

●      Packed Bed Adsorption (PBA) Chromatography Procedure (Detailed) in Flow-Through Mode

●      Expanded Bed Adsorption (EBA) Chromatography Procedure

●      Membrane Adsorption Procedure in Flow-Through Mode

●      Membrane Adsorption Procedure in Bind-and-Elute Mode