Expanded Bed Adsorption (EBA) Chromatography Procedure

Overview

An Expanded Bed Adsorption (EBA) Chromatography Procedure can be created by selecting the following menu option:
Unit Procedures } Chromatography/Adsorption } EBA Chromatography

	Operating Mode	Batch/Semi-Continuous
	Host Equipment	Expanded Bed Adsorption (EBA) Chromatography Column
	Emissions Port (Vent)	No
	Special Input/Output Ports	Yes

This unit procedure simulates any type of adsorptive chromatography (e.g., ion exchange, affinity, HIC, reverse phase, etc.) that takes place in a expanded bed column.

Special Inputs / Outputs

●      Waste: the designated output port for waste.

Operations

The following operation types are currently supported in the context of this unit procedure:

●      Load, see Expanded Bed Adsorption (EBA) Column Loading

●      Wash, see Column Wash (Simplified)

●      Elute, see Column Elution (Simplified)

●      Regenerate, see Column Regeneration (Simplified)

●      Equilibrate, see Column Equilibration (Simplified)

●      CIP, see Clean-In-Place (CIP)

●      Hold, see Holding

●      SIP, see Steam-In-Place (SIP)

SEe Also

The following procedures offer a similar functionality:

●      Gel Filtration Procedure

●      Packed Bed Adsorption (PBA) Chromatography Procedure (Simplified)

●      Packed Bed Adsorption (PBA) Chromatography Procedure (Detailed) in Bind-and-Elute Mode

●      Packed Bed Adsorption (PBA) Chromatography Procedure (Detailed) in Flow-Through Mode

●      Membrane Adsorption Procedure in Flow-Through Mode

●      Membrane Adsorption Procedure in Bind-and-Elute Mode