Gel Filtration Procedure

Overview

A Gel Filtration Procedure can be created by selecting the following menu option:
Unit Procedures } Chromatography/Adsorption } Gel Filtration

	Operating Mode	Batch/Semi-Continuous
	Host Equipment	Gel Filtration (GFL) Chromatography Column
	Emissions Port (Vent)	No
	Special Input/Output Ports	No

This unit procedure simulates purification of macromolecules using gel filtration, which is also known as size exclusion chromatography.

Special Inputs / Outputs

None.

Operations

The following operation types are currently supported in the context of this unit procedure:

●      Load, see Gel Filtration (GFL) Column Loading

●      Elute, see Column Elution (Simplified)

●      Wash, see Column Wash (Simplified)

●      Equilibrate, see Column Equilibration (Simplified)

●      Regenerate, see Column Regeneration (Simplified)

●      CIP, see Clean-In-Place (CIP)

●      Hold, see Holding

●      SIP, see Steam-In-Place (SIP)

SEe Also

The following procedures offer a similar functionality:

●      Packed Bed Adsorption (PBA) Chromatography Procedure (Simplified)

●      Packed Bed Adsorption (PBA) Chromatography Procedure (Detailed) in Bind-and-Elute Mode

●      Packed Bed Adsorption (PBA) Chromatography Procedure (Detailed) in Flow-Through Mode

●      Packed Bed Adsorption (PBA) Chromatography Procedure (Detailed) in Bind-and-Elute Mode

●      Packed Bed Adsorption (PBA) Chromatography Procedure (Detailed) in Flow-Through Mode

●      Expanded Bed Adsorption (EBA) Chromatography Procedure

●      Membrane Adsorption Procedure in Flow-Through Mode

●      Membrane Adsorption Procedure in Bind-and-Elute Mode