This operation simulates a column stripping step in flow-through chromatography which is used to remove any leftover impurities bound or stuck in the resin during the flow-through operation. The main objectives of this operation are to determine buffer consumption and account for the release of retained mass from the membrane.
● Packed Bed Adsorption (PBA) Chromatography Procedure (Detailed) in Flow-Through Mode
See Column Wash: Modeling Calculations.
The interface of this operation has the following tabs:
● Oper. Cond’s, see Column Wash: Oper. Conds Tab
● Labor, etc, see Operations Dialog: Labor etc. Tab
● Description, see Operations Dialog: Description Tab
● Batch Sheet, see Operations Dialog: Batch Sheet Tab
● Scheduling, see Operations Dialog: Scheduling Tab