Column Sanitization

General Description

This operation simulates a PBA column sanitization step in flow-through and bind-and-elute chromatographies during which any contaminants in the column are removed. The main objective of this operation is to determine bufffer consumption. Any change that may occur in the column’s retained mass (such as the removal of contaminants) during this step is ignored.

Unit Procedure Availability

●      Packed Bed Adsorption (PBA) Chromatography Procedure (Detailed) in Bind-and-Elute Mode

●      Packed Bed Adsorption (PBA) Chromatography Procedure (Detailed) in Flow-Through Mode

Column Sanitization: Modeling Calculations

See Column Flush: Modeling Calculations.

Column Sanitization: Interface

The interface of this operation has the following tabs:

●      Oper. Cond’s, see Column Flush: Oper. Conds Tab

●      Labor, etc, see Operations Dialog: Labor etc. Tab

●      Description, see Operations Dialog: Description Tab

●      Batch Sheet, see Operations Dialog: Batch Sheet Tab

●      Scheduling, see Operations Dialog: Scheduling Tab