This operation simulates a PBA column equilibration step in flow-through and bind-and-elute chromatographies. This step includes actions that prepare the column for loading such as buffer replacement, adjustment of pH, etc. The main objective of this operation is to determine bufffer consumption. Any change that may occur in the column’s retained mass during this step is ignored.
● Packed Bed Adsorption (PBA) Chromatography Procedure (Detailed) in Bind-and-Elute Mode
● Packed Bed Adsorption (PBA) Chromatography Procedure (Detailed) in Flow-Through Mode
See Column Flush: Modeling Calculations.
The interface of this operation has the following tabs:
● Oper. Cond’s, see Column Flush: Oper. Conds Tab
● Labor, etc, see Operations Dialog: Labor etc. Tab
● Description, see Operations Dialog: Description Tab
● Batch Sheet, see Operations Dialog: Batch Sheet Tab
● Scheduling, see Operations Dialog: Scheduling Tab