GFL Chromatography Column: Equipment Tab

Variable Definitions, Ranges and Default Values

The following table shows the meaning of the variables appearing in this tab, as well as their default values and their generally acceptable range. Note that due to the manner by which the variables are used by the sizing and costing equations, the range of acceptable values may be further reduced.

Variable	Default Value	Range

◙ Bed Height The bed height of the column.	0.3 m	Positive
◙ Max Diameter The maximum diameter the column may have.	1.8 m	Positive
◙ Diameter The diameter of the column.	0.2 m	Positive
◙ Max Height The maximum bed height of the column.	1.0 m	Positive
◙ Column Height The total height of the column.	1.0 m	Positive
◙ Bed To Column Height The bed height over the column height ratio.	1.0	Positive
◙ Overdesign Factor Use a factor greater than 1.0 to design a larger column than that required by the corresponding operation. Used only in design mode.	1.0	Positive
● Bed Volume The volume of the bed of the column.	0.0 L	Positive.
● Column Volume The total volume of column.	0.0 L	Positive

Symbol Key: ○ User-specified value (always input); ● Calculated value (always output); ◙ Sometimes input, sometimes output

Consumables

The Gel Filtration Chromatography column requires the use of a consumable. By default the selected consumable is a ‘Dft Gel Filtration Resin’. The user may select the desired consumable for the column from the Chromatography/Adsorption Equipment: Consumables tab.

Sizing: Design Mode

In design mode, the column volume demand is specified by the Gel Filtration loading operations carried out as part of the (one or more) gel filtration unit procedures hosted by the equipment resource. The software will size the column based on the largest demand on column volume amongst all ion exchange loading operations executed in the column. Once the largest column volume demand is determined, it is multiplied by the overdesign factor to determine the total column volume requirement. This is used to calculate the corresponding bed diameter and column height based on the specifications for the bed height and the bed-to-column height. If the calculated bed diameter exceeds the maximum bed diameter specification then the software will assume multiple number of identical units (each with column volume equally distributed) that are operated in parallel. From the column volume per unit, the actual bed diameter and bed volume of each column are calculated. In addition, if the bed height is specified, the bed height to diameter ratio is also calculated.

Sizing: Rating Mode

In User-Defined (Rating Mode), the user specifies the Number of Units, the Bed Height, Diameter, and Column Height. If the column is undersized for its separation task, the program displays a warning advising the user to increase the number of cycles per batch or use a larger column.

Costing

The equipment purchase cost is estimated using built-in correlations derived from vendor data. The equipment cost is a function of column dimensions, material of construction, and throughput.

For more information on this equipment, see Gel Filtration (GFL) Chromatography Column.

For information on the left-hand side variables and options, see Equipment Data Dialog: Equipment tab.