High Pressure Homogenization: Mat. Balance Tab

Variable Definitions, Ranges and Default Values

The following table shows a brief description of the variables appearing in this tab. The table also displays their default values and their generally acceptable range:

Variable	Default Value	Range

○ Mass Coeff. The homogenization mass stoichiometric coefficient of the corresponding component.	0.0	Positive
○ Extra-Cell. (%) The extra-cellular mass percentage of the corresponding product component.	100	0 – 100
○ Alpha Constant of the homogenization kinetic reaction.	2.9	Positive
○ k Kinetic constant of the homogenization kinetic reaction.	1.0e-8	Positive
○ Phi Kinetic constant of the homogenization kinetic reaction.	0.01	Positive
○ Release (%)	0.0	Positive
○ Denaturation (%)	0.0	Positive
○ Active Product Indicates the active product component. Click on the list box to bring up a list of available components in order to select one.	<none>	Any Pure Component
○ Denatured Product Component Indicates the denatured product component. Click on the list box to bring up a list of available components in order to select one.	<none>	Any Pure Component

Symbol Key: ○ User-specified value (always input); ● Calculated value (always output); ◙ Sometimes input, sometimes output

Specification Choices / Comments

The following list describes the available specification choices in this tab; for more details on how these are implemented, see High Pressure Homogenization: Modeling Calculations.

Adding /Deleting Reactants and Products

Clicking on the button in the reactants / products displays a list of available reactants / products that can be added to the reaction. Select a component to add it either as a reactant or a product. You can alter the stoichiometric coefficient of the component through the respective table (Reactants/Products). Note that if a component is used as a reactant it can not be used as a product and vice versa. To delete a reactant or a product, first select it by clicking on the corresponding row number, and then click on the button.

Stoichiometric Coefficients

Use positive coefficients for both products and reactants. The total mass of reactants is displayed below the reactants table and the total mass of products is displayed below the products table. The total mass of reactants must be equal to the total mass of products.

Extra-Cell (%)

This feature is useful when tracking of intracellular water is desired because it affects the performance of centrifugation, filtration, etc. further downstream. If the ‘Extra-Cell %’ of certain product components is less than 100 (or in other words a fraction of the component is intracellular) and the ‘Primary Biomass’ and ‘Water’ components are identified (through the Pure Component Registration Dialog window), then the model automatically associates intracellular water with the intracellular reaction product components. Then, if a separator is used to remove intracellular components (i.e., removal of biomass by a centrifuge), the separation (removal %) of intracellular water will be the same as that of the intracellular component(s).

Homogenization (Cell Disruption) Kinetics Options...

You can specify either the kinetic constants or the results (release and denaturation) of the homogenization (cell disruption) reaction.

Homogenization of Food Products...

In this case, simply specify the pressure drop through the valve so that the model can calculate the power requirement. There is no need to specify a reaction stoichiometry since no material transformation takes place.